A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data
نویسندگان
چکیده
منابع مشابه
A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data
BACKGROUND Quantitative PCR (qPCR) is a workhorse laboratory technique for measuring the concentration of a target DNA sequence with high accuracy over a wide dynamic range. The gold standard method for estimating DNA concentrations via qPCR is quantification cycle () standard curve quantification, which requires the time- and labor-intensive construction of a standard curve. In theory, the sha...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2010
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0012355